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KMID : 0363320170380060891
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Journal of Korean Oriental Internal Medicine
2017 Volume.38 No. 6 p.891 ~ p.901
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Anti-asthmatic Effect of Alismatis Rhizoma and Alisol Acetate B Combination Therapy in a Murine Asthma Model
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Park Mi-Jun
Heo June-Yi
Kwun Min-Jung
Han Chang-Woo
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Abstract
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Objectives: The aim of the study was to evaluate the anti-asthmatic effect of alismatis rhizoma and alisol acetate B combination therapy in a murine asthma model.
Methods: C57BL/6 mice were sensitized to and challenged with a mixture of ragweed, dust mite, and aspergillus to induce an asthma animal model. Alismatis rhizoma extract and alisol acetate B combination therapy was co-administered only in the experimental group. To evaluate the anti-asthmatic effect of the combination therapy, inflammatory cell counts in bronchoalveolar lavage (BAL) fluid were determined, and tissue was examined histologically with hematoxylin and eosin (H & E) and periodic acid-Schiff (PAS) stains, by enzyme-linked immunosorbent assay (ELISA) of IgE, IL-4, and IL-5, and with reverse transcription polymerase chain reaction (RT-PCR) of IL-5, IL-33, MUC5AC.
Results: Alismatis rhizoma and alisol acetate B combination therapy reduced the number of inflammatory cells, alleviated histologic features, and down-regulated all the investigated asthma mediators, IgE, IL-4, IL-5, IL-33, and MUC5AC.
Conclusions: According to the above results, alismatis rhizoma and alisol acetate B combination therapy may have therapeutic potential for asthma.
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KEYWORD
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Alismatis rhizoma (Alisma orientale), alisol acetate B, asthma
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